Preparation of scFv stabilized chromatosomes for single-particle cryo-EM structure determination

The chromatosome, a nucleosome bound to a histone H1, is the structural unit of metazoan chromatin. Determination of the high-resolution structure of the chromatosome is challenging due to the dynamic nature of H1 binding. Here, we present a protocol for purifying an optimized single-chain antibody...

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Detalles Bibliográficos
Autores principales: Zhou, Bing-Rui, Bai, Yawen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994535/
https://www.ncbi.nlm.nih.gov/pubmed/33786462
http://dx.doi.org/10.1016/j.xpro.2021.100396

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994535/
https://www.ncbi.nlm.nih.gov/pubmed/33786462
http://dx.doi.org/10.1016/j.xpro.2021.100396

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