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Preparation of scFv stabilized chromatosomes for single-particle cryo-EM structure determination
The chromatosome, a nucleosome bound to a histone H1, is the structural unit of metazoan chromatin. Determination of the high-resolution structure of the chromatosome is challenging due to the dynamic nature of H1 binding. Here, we present a protocol for purifying an optimized single-chain antibody...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994535/ https://www.ncbi.nlm.nih.gov/pubmed/33786462 http://dx.doi.org/10.1016/j.xpro.2021.100396 |