An RNA in situ hybridization protocol optimized for monocot tissue

RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probe...

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Detalles Bibliográficos
Autores principales: Zöllner, Nora R., Bezrutczyk, Margaret, Laureyns, Reinout, Nelissen, Hilde, Simon, Rüdiger, Frommer, Wolf B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://www.ncbi.nlm.nih.gov/pubmed/33796873
http://dx.doi.org/10.1016/j.xpro.2021.100398

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://www.ncbi.nlm.nih.gov/pubmed/33796873
http://dx.doi.org/10.1016/j.xpro.2021.100398

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