An RNA in situ hybridization protocol optimized for monocot tissue

RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probe...

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Detalles Bibliográficos
Autores principales: Zöllner, Nora R., Bezrutczyk, Margaret, Laureyns, Reinout, Nelissen, Hilde, Simon, Rüdiger, Frommer, Wolf B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://www.ncbi.nlm.nih.gov/pubmed/33796873
http://dx.doi.org/10.1016/j.xpro.2021.100398
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author Zöllner, Nora R.
Bezrutczyk, Margaret
Laureyns, Reinout
Nelissen, Hilde
Simon, Rüdiger
Frommer, Wolf B.
author_facet Zöllner, Nora R.
Bezrutczyk, Margaret
Laureyns, Reinout
Nelissen, Hilde
Simon, Rüdiger
Frommer, Wolf B.
author_sort Zöllner, Nora R.
collection PubMed
description RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections. For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021).
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spelling pubmed-79956562021-03-31 An RNA in situ hybridization protocol optimized for monocot tissue Zöllner, Nora R. Bezrutczyk, Margaret Laureyns, Reinout Nelissen, Hilde Simon, Rüdiger Frommer, Wolf B. STAR Protoc Protocol RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections. For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021). Elsevier 2021-03-17 /pmc/articles/PMC7995656/ /pubmed/33796873 http://dx.doi.org/10.1016/j.xpro.2021.100398 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zöllner, Nora R.
Bezrutczyk, Margaret
Laureyns, Reinout
Nelissen, Hilde
Simon, Rüdiger
Frommer, Wolf B.
An RNA in situ hybridization protocol optimized for monocot tissue
title An RNA in situ hybridization protocol optimized for monocot tissue
title_full An RNA in situ hybridization protocol optimized for monocot tissue
title_fullStr An RNA in situ hybridization protocol optimized for monocot tissue
title_full_unstemmed An RNA in situ hybridization protocol optimized for monocot tissue
title_short An RNA in situ hybridization protocol optimized for monocot tissue
title_sort rna in situ hybridization protocol optimized for monocot tissue
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995656/
https://www.ncbi.nlm.nih.gov/pubmed/33796873
http://dx.doi.org/10.1016/j.xpro.2021.100398
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