Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn(2+) in the Golgi apparatus in live cells

Quantitative analysis using a turn-on fluorescent probe is inherently difficult due to the dependency of the fluorescence intensity on the probe concentration. To overcome this limitation, we developed an in situ quantification method using a turn-on fluorescent probe and a standard fluorophore, whi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kowada, Toshiyuki, Watanabe, Tomomi, Liu, Rong, Mizukami, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995662/
https://www.ncbi.nlm.nih.gov/pubmed/33796872
http://dx.doi.org/10.1016/j.xpro.2021.100395

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995662/
https://www.ncbi.nlm.nih.gov/pubmed/33796872
http://dx.doi.org/10.1016/j.xpro.2021.100395

Ejemplares similares