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Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn(2+) in the Golgi apparatus in live cells
Quantitative analysis using a turn-on fluorescent probe is inherently difficult due to the dependency of the fluorescence intensity on the probe concentration. To overcome this limitation, we developed an in situ quantification method using a turn-on fluorescent probe and a standard fluorophore, whi...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995662/ https://www.ncbi.nlm.nih.gov/pubmed/33796872 http://dx.doi.org/10.1016/j.xpro.2021.100395 |