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Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

BACKGROUND: SARS-CoV-2 Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) colorimetric detection is a sensitive and specific point-of-care molecular biology technique used to detect the virus in only 30 min. In this manuscript we have described a few nuances of the technique stil...

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Detalles Bibliográficos
Autores principales: de Oliveira Coelho, Bruna, Sanchuki, Heloisa Bruna Soligo, Zanette, Dalila Luciola, Nardin, Jeanine Marie, Morales, Hugo Manuel Paz, Fornazari, Bruna, Aoki, Mateus Nóbrega, Blanes, Lucas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996115/
https://www.ncbi.nlm.nih.gov/pubmed/33771097
http://dx.doi.org/10.1186/s10020-021-00289-0