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Novel Template Plasmids pCyaA’-Kan and pCyaA’-Cam for Generation of Unmarked Chromosomal cyaA’ Translational Fusion to T3SS Effectors in Salmonella

The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of Salmonella. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the functionality of T3SS effectors, gene fusions to the CyaA’...

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Detalles Bibliográficos
Autores principales: Fernández, Paulina A., Zabner, Marcela, Ortega, Jaime, Morgado, Constanza, Amaya, Fernando, Vera, Gabriel, Rubilar, Carolina, Salas, Beatriz, Cuevas, Víctor, Valenzuela, Camila, Baisón-Olmo, Fernando, Álvarez, Sergio A., Santiviago, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996335/
https://www.ncbi.nlm.nih.gov/pubmed/33668764
http://dx.doi.org/10.3390/microorganisms9030475