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A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression

The cell-free protein synthesis (CFPS) that synthesizes mRNA and protein from a template DNA has been featured as an important tool to emulate living systems in vitro. However, an obstacle to emulate living cells by CFPS is the loss of activity in the case of usage of high concentration cell extract...

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Detalles Bibliográficos
Autores principales: Takahashi, Katsuki, Sato, Gaku, Doi, Nobuhide, Fujiwara, Kei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999496/
https://www.ncbi.nlm.nih.gov/pubmed/33805612
http://dx.doi.org/10.3390/life11030237