Cargando…
A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression
The cell-free protein synthesis (CFPS) that synthesizes mRNA and protein from a template DNA has been featured as an important tool to emulate living systems in vitro. However, an obstacle to emulate living cells by CFPS is the loss of activity in the case of usage of high concentration cell extract...
Autores principales: | Takahashi, Katsuki, Sato, Gaku, Doi, Nobuhide, Fujiwara, Kei |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999496/ https://www.ncbi.nlm.nih.gov/pubmed/33805612 http://dx.doi.org/10.3390/life11030237 |
Ejemplares similares
-
Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption
por: Fujiwara, Kei, et al.
Publicado: (2016) -
Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration
por: Matsui, Yukino, et al.
Publicado: (2021) -
System concentration shift as a regulator of transcription-translation system within liposomes
por: Akui, Toshiki, et al.
Publicado: (2021) -
Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system
por: Yoshida, Aoi, et al.
Publicado: (2019) -
Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells
por: Kohyama, Shunshi, et al.
Publicado: (2019)