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Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts

The poor prognosis of locally advanced and metastatic head and neck squamous cell carcinoma (HNSCC) is primarily mediated by the functional properties of cancer stem cells (CSCs) and resistance to chemoradiotherapy. We investigated whether the aldehyde dehydrogenase (ALDH) inhibitor disulfiram (DSF)...

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Autores principales: Yao, Wenhao, Qian, Xu, Ochsenreither, Sebastian, Soldano, Ferrone, DeLeo, Albert B., Sudhoff, Holger, Oppel, Felix, Kuppig, Andreas, Klinghammer, Konrad, Kaufmann, Andreas M., Albers, Andreas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999545/
https://www.ncbi.nlm.nih.gov/pubmed/33671083
http://dx.doi.org/10.3390/cells10030517
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author Yao, Wenhao
Qian, Xu
Ochsenreither, Sebastian
Soldano, Ferrone
DeLeo, Albert B.
Sudhoff, Holger
Oppel, Felix
Kuppig, Andreas
Klinghammer, Konrad
Kaufmann, Andreas M.
Albers, Andreas E.
author_facet Yao, Wenhao
Qian, Xu
Ochsenreither, Sebastian
Soldano, Ferrone
DeLeo, Albert B.
Sudhoff, Holger
Oppel, Felix
Kuppig, Andreas
Klinghammer, Konrad
Kaufmann, Andreas M.
Albers, Andreas E.
author_sort Yao, Wenhao
collection PubMed
description The poor prognosis of locally advanced and metastatic head and neck squamous cell carcinoma (HNSCC) is primarily mediated by the functional properties of cancer stem cells (CSCs) and resistance to chemoradiotherapy. We investigated whether the aldehyde dehydrogenase (ALDH) inhibitor disulfiram (DSF) can enhance the sensitivity of therapy. Cell viability was assessed by the 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) and apoptosis assays, and the cell cycle and reactive oxygen species (ROS) levels were evaluated by fluorescence-activated cell sorting (FACS). The radio-sensitizing effect was measured by a colony formation assay. The synergistic effects were calculated by combination index (CI) analyses. The DSF and DSF/Cu(2+) inhibited the cell proliferation (inhibitory concentration 50 (IC(50)) of DSF and DSF/Cu(2+) were 13.96 μM and 0.24 μM). DSF and cisplatin displayed a synergistic effect (CI values were <1). DSF or DSF/Cu(2+) abolished the cisplatin-induced G2/M arrest (from 52.9% to 40.7% and 41.1%), and combining irradiation (IR) with DSF or DSF/Cu(2+) reduced the colony formation and attenuated the G2/M arrest (from 53.6% to 40.2% and 41.9%). The combination of cisplatin, DSF or DSF/Cu(2+), and IR enhanced the radio-chemo sensitivity by inducing apoptosis (42.04% and 32.21%) and ROS activity (46.3% and 37.4%). DSF and DSF/Cu(2+) enhanced the sensitivity of HNSCC to cisplatin and IR. Confirming the initial data from patient-derived tumor xenograft (PDX) supported a strong rationale to repurpose DSF as a radio-chemosensitizer and to assess its therapeutic potential in a clinical setting.
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spelling pubmed-79995452021-03-28 Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts Yao, Wenhao Qian, Xu Ochsenreither, Sebastian Soldano, Ferrone DeLeo, Albert B. Sudhoff, Holger Oppel, Felix Kuppig, Andreas Klinghammer, Konrad Kaufmann, Andreas M. Albers, Andreas E. Cells Article The poor prognosis of locally advanced and metastatic head and neck squamous cell carcinoma (HNSCC) is primarily mediated by the functional properties of cancer stem cells (CSCs) and resistance to chemoradiotherapy. We investigated whether the aldehyde dehydrogenase (ALDH) inhibitor disulfiram (DSF) can enhance the sensitivity of therapy. Cell viability was assessed by the 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) and apoptosis assays, and the cell cycle and reactive oxygen species (ROS) levels were evaluated by fluorescence-activated cell sorting (FACS). The radio-sensitizing effect was measured by a colony formation assay. The synergistic effects were calculated by combination index (CI) analyses. The DSF and DSF/Cu(2+) inhibited the cell proliferation (inhibitory concentration 50 (IC(50)) of DSF and DSF/Cu(2+) were 13.96 μM and 0.24 μM). DSF and cisplatin displayed a synergistic effect (CI values were <1). DSF or DSF/Cu(2+) abolished the cisplatin-induced G2/M arrest (from 52.9% to 40.7% and 41.1%), and combining irradiation (IR) with DSF or DSF/Cu(2+) reduced the colony formation and attenuated the G2/M arrest (from 53.6% to 40.2% and 41.9%). The combination of cisplatin, DSF or DSF/Cu(2+), and IR enhanced the radio-chemo sensitivity by inducing apoptosis (42.04% and 32.21%) and ROS activity (46.3% and 37.4%). DSF and DSF/Cu(2+) enhanced the sensitivity of HNSCC to cisplatin and IR. Confirming the initial data from patient-derived tumor xenograft (PDX) supported a strong rationale to repurpose DSF as a radio-chemosensitizer and to assess its therapeutic potential in a clinical setting. MDPI 2021-02-28 /pmc/articles/PMC7999545/ /pubmed/33671083 http://dx.doi.org/10.3390/cells10030517 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Yao, Wenhao
Qian, Xu
Ochsenreither, Sebastian
Soldano, Ferrone
DeLeo, Albert B.
Sudhoff, Holger
Oppel, Felix
Kuppig, Andreas
Klinghammer, Konrad
Kaufmann, Andreas M.
Albers, Andreas E.
Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title_full Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title_fullStr Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title_full_unstemmed Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title_short Disulfiram Acts as a Potent Radio-Chemo Sensitizer in Head and Neck Squamous Cell Carcinoma Cell Lines and Transplanted Xenografts
title_sort disulfiram acts as a potent radio-chemo sensitizer in head and neck squamous cell carcinoma cell lines and transplanted xenografts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999545/
https://www.ncbi.nlm.nih.gov/pubmed/33671083
http://dx.doi.org/10.3390/cells10030517
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