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Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis

The recovery of amino acids and other important bioactive compounds from the comb penshell (Atrina pectinata) using subcritical water hydrolysis was performed. A wide range of extraction temperatures from 140 to 290 °C was used to evaluate the release of proteins and amino acids. The amount of crude...

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Autores principales: Lee, Hee-Jeong, Roy, Vikash Chandra, Ho, Truc Cong, Park, Jin-Seok, Jeong, Yu-Rin, Lee, Seung-Chan, Kim, Sung-Yeol, Chun, Byung-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999596/
https://www.ncbi.nlm.nih.gov/pubmed/33804423
http://dx.doi.org/10.3390/md19030137
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author Lee, Hee-Jeong
Roy, Vikash Chandra
Ho, Truc Cong
Park, Jin-Seok
Jeong, Yu-Rin
Lee, Seung-Chan
Kim, Sung-Yeol
Chun, Byung-Soo
author_facet Lee, Hee-Jeong
Roy, Vikash Chandra
Ho, Truc Cong
Park, Jin-Seok
Jeong, Yu-Rin
Lee, Seung-Chan
Kim, Sung-Yeol
Chun, Byung-Soo
author_sort Lee, Hee-Jeong
collection PubMed
description The recovery of amino acids and other important bioactive compounds from the comb penshell (Atrina pectinata) using subcritical water hydrolysis was performed. A wide range of extraction temperatures from 140 to 290 °C was used to evaluate the release of proteins and amino acids. The amount of crude protein was the highest (36.14 ± 1.39 mg bovine serum albumin/g) at 200 °C, whereas a further increase in temperature showed the degradation of the crude protein content. The highest amount of amino acids (74.80 mg/g) was at 230 °C, indicating that the temperature range of 170–230 °C is suitable for the extraction of protein-rich compounds using subcritical water hydrolysis. Molecular weights of the peptides obtained from comb penshell viscera decreased with the increasing temperature. SDS-PAGE revealed that the molecular weight of peptides present in the hydrolysates above the 200 °C extraction temperature was ≤ 1000 Da. Radical scavenging activities were analyzed to evaluate the antioxidant activities of the hydrolysates. A. pectinata hydrolysates also showed a particularly good antihypertensive activity, proving that this raw material can be an effective source of amino acids and marine bioactive peptides.
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spelling pubmed-79995962021-03-28 Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis Lee, Hee-Jeong Roy, Vikash Chandra Ho, Truc Cong Park, Jin-Seok Jeong, Yu-Rin Lee, Seung-Chan Kim, Sung-Yeol Chun, Byung-Soo Mar Drugs Article The recovery of amino acids and other important bioactive compounds from the comb penshell (Atrina pectinata) using subcritical water hydrolysis was performed. A wide range of extraction temperatures from 140 to 290 °C was used to evaluate the release of proteins and amino acids. The amount of crude protein was the highest (36.14 ± 1.39 mg bovine serum albumin/g) at 200 °C, whereas a further increase in temperature showed the degradation of the crude protein content. The highest amount of amino acids (74.80 mg/g) was at 230 °C, indicating that the temperature range of 170–230 °C is suitable for the extraction of protein-rich compounds using subcritical water hydrolysis. Molecular weights of the peptides obtained from comb penshell viscera decreased with the increasing temperature. SDS-PAGE revealed that the molecular weight of peptides present in the hydrolysates above the 200 °C extraction temperature was ≤ 1000 Da. Radical scavenging activities were analyzed to evaluate the antioxidant activities of the hydrolysates. A. pectinata hydrolysates also showed a particularly good antihypertensive activity, proving that this raw material can be an effective source of amino acids and marine bioactive peptides. MDPI 2021-03-01 /pmc/articles/PMC7999596/ /pubmed/33804423 http://dx.doi.org/10.3390/md19030137 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Lee, Hee-Jeong
Roy, Vikash Chandra
Ho, Truc Cong
Park, Jin-Seok
Jeong, Yu-Rin
Lee, Seung-Chan
Kim, Sung-Yeol
Chun, Byung-Soo
Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title_full Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title_fullStr Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title_full_unstemmed Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title_short Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis
title_sort amino acid profiles and biopotentiality of hydrolysates obtained from comb penshell (atrina pectinata) viscera using subcritical water hydrolysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999596/
https://www.ncbi.nlm.nih.gov/pubmed/33804423
http://dx.doi.org/10.3390/md19030137
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