Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies

A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered...

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Detalles Bibliográficos
Autores principales: Carratalá, Jose Vicente, Gifre-Renom, Laia, Roca-Pinilla, Ramon, Villaverde, Antonio, Arís, Anna, Garcia-Fruitós, Elena, Sánchez, Julieta María, Ferrer-Miralles, Neus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001920/
https://www.ncbi.nlm.nih.gov/pubmed/33809594
http://dx.doi.org/10.3390/ijms22063020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001920/
https://www.ncbi.nlm.nih.gov/pubmed/33809594
http://dx.doi.org/10.3390/ijms22063020

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