RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in p...

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Detalles Bibliográficos
Autores principales: Shittu, Mujeeb, Steenwinkel, Tessa, Dion, William, Ostlund, Nathan, Raja, Komal, Werner, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005959/
https://www.ncbi.nlm.nih.gov/pubmed/33801802
http://dx.doi.org/10.3390/mps4010020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005959/
https://www.ncbi.nlm.nih.gov/pubmed/33801802
http://dx.doi.org/10.3390/mps4010020

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