RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in p...

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Autores principales: Shittu, Mujeeb, Steenwinkel, Tessa, Dion, William, Ostlund, Nathan, Raja, Komal, Werner, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005959/
https://www.ncbi.nlm.nih.gov/pubmed/33801802
http://dx.doi.org/10.3390/mps4010020
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author Shittu, Mujeeb
Steenwinkel, Tessa
Dion, William
Ostlund, Nathan
Raja, Komal
Werner, Thomas
author_facet Shittu, Mujeeb
Steenwinkel, Tessa
Dion, William
Ostlund, Nathan
Raja, Komal
Werner, Thomas
author_sort Shittu, Mujeeb
collection PubMed
description RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.
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spelling pubmed-80059592021-03-30 RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species Shittu, Mujeeb Steenwinkel, Tessa Dion, William Ostlund, Nathan Raja, Komal Werner, Thomas Methods Protoc Protocol RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species. MDPI 2021-03-10 /pmc/articles/PMC8005959/ /pubmed/33801802 http://dx.doi.org/10.3390/mps4010020 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Shittu, Mujeeb
Steenwinkel, Tessa
Dion, William
Ostlund, Nathan
Raja, Komal
Werner, Thomas
RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title_full RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title_fullStr RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title_full_unstemmed RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title_short RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
title_sort rna in situ hybridization for detecting gene expression patterns in the abdomens and wings of drosophila species
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005959/
https://www.ncbi.nlm.nih.gov/pubmed/33801802
http://dx.doi.org/10.3390/mps4010020
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