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RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species
RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in p...
Autores principales: | Shittu, Mujeeb, Steenwinkel, Tessa, Dion, William, Ostlund, Nathan, Raja, Komal, Werner, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005959/ https://www.ncbi.nlm.nih.gov/pubmed/33801802 http://dx.doi.org/10.3390/mps4010020 |
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