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The effect of methyl-beta-cyclodextrin on DNA absorption and quality of posttransfected sperm

Sperm can be selected as a natural vector for the production of transgenic animals. Methyl-beta-cyclodextrin (MBCD) removes cholesterol from the phospholipid membrane of sperm and improves the efficiency of DNA uptake by sperm. In experiment 1, fresh sperm was treated with various concentrations of...

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Detalles Bibliográficos
Autores principales: Rahimi, Mahin, Rahimi, Shaban, Sharafi, Mohsen, Shahverdi, Abdolhossein, Grimes, J.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010517/
https://www.ncbi.nlm.nih.gov/pubmed/33743498
http://dx.doi.org/10.1016/j.psj.2021.101058