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The effect of methyl-beta-cyclodextrin on DNA absorption and quality of posttransfected sperm
Sperm can be selected as a natural vector for the production of transgenic animals. Methyl-beta-cyclodextrin (MBCD) removes cholesterol from the phospholipid membrane of sperm and improves the efficiency of DNA uptake by sperm. In experiment 1, fresh sperm was treated with various concentrations of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010517/ https://www.ncbi.nlm.nih.gov/pubmed/33743498 http://dx.doi.org/10.1016/j.psj.2021.101058 |