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Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

BACKGROUND: Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the high temperatures during industrial steam pelleting. Increasing thermostability o...

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Detalles Bibliográficos
Autores principales: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos H., Chen, Xiaojing, von Hellens, Juhani, Mahler, Stephen, Speight, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010977/
https://www.ncbi.nlm.nih.gov/pubmed/33789740
http://dx.doi.org/10.1186/s13068-021-01936-8