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Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
BACKGROUND: Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the high temperatures during industrial steam pelleting. Increasing thermostability o...
Autores principales: | Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos H., Chen, Xiaojing, von Hellens, Juhani, Mahler, Stephen, Speight, Robert |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010977/ https://www.ncbi.nlm.nih.gov/pubmed/33789740 http://dx.doi.org/10.1186/s13068-021-01936-8 |
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