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Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension

Genome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeri...

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Autores principales: Mengoli, Valentina, Jonak, Katarzyna, Lyzak, Oleksii, Lamb, Mahdi, Lister, Lisa M, Lodge, Chris, Rojas, Julie, Zagoriy, Ievgeniia, Herbert, Mary, Zachariae, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013787/
https://www.ncbi.nlm.nih.gov/pubmed/33644894
http://dx.doi.org/10.15252/embj.2020106812
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author Mengoli, Valentina
Jonak, Katarzyna
Lyzak, Oleksii
Lamb, Mahdi
Lister, Lisa M
Lodge, Chris
Rojas, Julie
Zagoriy, Ievgeniia
Herbert, Mary
Zachariae, Wolfgang
author_facet Mengoli, Valentina
Jonak, Katarzyna
Lyzak, Oleksii
Lamb, Mahdi
Lister, Lisa M
Lodge, Chris
Rojas, Julie
Zagoriy, Ievgeniia
Herbert, Mary
Zachariae, Wolfgang
author_sort Mengoli, Valentina
collection PubMed
description Genome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeric cohesin by shugoshin‐PP2A seems evolutionarily conserved. However, deprotection has been proposed to rely on spindle forces separating the Rec8 protector from cohesin at metaphase II in mammalian oocytes and on APC/C‐dependent destruction of the protector at anaphase II in yeast. Here, we have activated APC/C in the absence of sister kinetochore biorientation at meiosis II in yeast and mouse oocytes, and find that bipolar spindle forces are dispensable for sister centromere separation in both systems. Furthermore, we show that at least in yeast, protection of Rec8 by shugoshin and inhibition of separase by securin are both required for the stability of centromeric cohesin at metaphase II. Our data imply that related mechanisms preserve the integrity of dyad chromosomes during the short metaphase II of yeast and the prolonged metaphase II arrest of mammalian oocytes.
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spelling pubmed-80137872021-04-02 Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension Mengoli, Valentina Jonak, Katarzyna Lyzak, Oleksii Lamb, Mahdi Lister, Lisa M Lodge, Chris Rojas, Julie Zagoriy, Ievgeniia Herbert, Mary Zachariae, Wolfgang EMBO J Articles Genome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeric cohesin by shugoshin‐PP2A seems evolutionarily conserved. However, deprotection has been proposed to rely on spindle forces separating the Rec8 protector from cohesin at metaphase II in mammalian oocytes and on APC/C‐dependent destruction of the protector at anaphase II in yeast. Here, we have activated APC/C in the absence of sister kinetochore biorientation at meiosis II in yeast and mouse oocytes, and find that bipolar spindle forces are dispensable for sister centromere separation in both systems. Furthermore, we show that at least in yeast, protection of Rec8 by shugoshin and inhibition of separase by securin are both required for the stability of centromeric cohesin at metaphase II. Our data imply that related mechanisms preserve the integrity of dyad chromosomes during the short metaphase II of yeast and the prolonged metaphase II arrest of mammalian oocytes. John Wiley and Sons Inc. 2021-03-01 2021-04-01 /pmc/articles/PMC8013787/ /pubmed/33644894 http://dx.doi.org/10.15252/embj.2020106812 Text en © 2021 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Mengoli, Valentina
Jonak, Katarzyna
Lyzak, Oleksii
Lamb, Mahdi
Lister, Lisa M
Lodge, Chris
Rojas, Julie
Zagoriy, Ievgeniia
Herbert, Mary
Zachariae, Wolfgang
Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title_full Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title_fullStr Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title_full_unstemmed Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title_short Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
title_sort deprotection of centromeric cohesin at meiosis ii requires apc/c activity but not kinetochore tension
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013787/
https://www.ncbi.nlm.nih.gov/pubmed/33644894
http://dx.doi.org/10.15252/embj.2020106812
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