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Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers

We previously introduced the use of DNA molecules for calibration of biophysical force and displacement measurements with optical tweezers. Force and length scale factors can be determined from measurements of DNA stretching. Trap compliance can be determined by fitting the data to a nonlinear DNA e...

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Detalles Bibliográficos
Autores principales: Mo, Youbin, Fizari, Mounir, Koharchik, Kristina, Smith, Douglas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019724/
https://www.ncbi.nlm.nih.gov/pubmed/33829038
http://dx.doi.org/10.3389/fmolb.2021.605102