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Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems. Non-replicating plasmids are currently employed to achieve insertional mutagenesis in Bifidobacterium. One of the limita...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021953/ https://www.ncbi.nlm.nih.gov/pubmed/33833740 http://dx.doi.org/10.3389/fmicb.2021.636822 |