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Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve

Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems. Non-replicating plasmids are currently employed to achieve insertional mutagenesis in Bifidobacterium. One of the limita...

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Detalles Bibliográficos
Autores principales:	Hoedt, Emily C., Bottacini, Francesca, Cash, Nora, Bongers, Roger S., van Limpt, Kees, Ben Amor, Kaouther, Knol, Jan, MacSharry, John, van Sinderen, Douwe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021953/ https://www.ncbi.nlm.nih.gov/pubmed/33833740 http://dx.doi.org/10.3389/fmicb.2021.636822

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