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A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to id...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024771/ https://www.ncbi.nlm.nih.gov/pubmed/33855304 http://dx.doi.org/10.1016/j.xpro.2021.100392 |