A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to id...

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Detalles Bibliográficos
Autores principales: Li, Feng, Hung, Stevephen, Esnault, Caroline, Levin, Henry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024771/
https://www.ncbi.nlm.nih.gov/pubmed/33855304
http://dx.doi.org/10.1016/j.xpro.2021.100392
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author Li, Feng
Hung, Stevephen
Esnault, Caroline
Levin, Henry L.
author_facet Li, Feng
Hung, Stevephen
Esnault, Caroline
Levin, Henry L.
author_sort Li, Feng
collection PubMed
description Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to identify genes necessary for the formation of centromeric heterochromatin. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020).
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spelling pubmed-80247712021-04-13 A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin Li, Feng Hung, Stevephen Esnault, Caroline Levin, Henry L. STAR Protoc Protocol Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to identify genes necessary for the formation of centromeric heterochromatin. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020). Elsevier 2021-03-23 /pmc/articles/PMC8024771/ /pubmed/33855304 http://dx.doi.org/10.1016/j.xpro.2021.100392 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Feng
Hung, Stevephen
Esnault, Caroline
Levin, Henry L.
A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title_full A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title_fullStr A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title_full_unstemmed A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title_short A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
title_sort protocol for transposon insertion sequencing in schizosaccharomyces pombe to identify factors that maintain heterochromatin
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024771/
https://www.ncbi.nlm.nih.gov/pubmed/33855304
http://dx.doi.org/10.1016/j.xpro.2021.100392
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