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Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming
Neural cell diversity is essential to endow distinct brain regions with specific functions. During development, progenitors within these regions are characterized by specific gene expression programs, contributing to the generation of diversity in postmitotic neurons and astrocytes. While the region...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026135/ https://www.ncbi.nlm.nih.gov/pubmed/33827819 http://dx.doi.org/10.1126/sciadv.abe8978 |
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author | Herrero-Navarro, Álvaro Puche-Aroca, Lorenzo Moreno-Juan, Verónica Sempere-Ferràndez, Alejandro Espinosa, Ana Susín, Rafael Torres-Masjoan, Laia Leyva-Díaz, Eduardo Karow, Marisa Figueres-Oñate, María López-Mascaraque, Laura López-Atalaya, José P. Berninger, Benedikt López-Bendito, Guillermina |
author_facet | Herrero-Navarro, Álvaro Puche-Aroca, Lorenzo Moreno-Juan, Verónica Sempere-Ferràndez, Alejandro Espinosa, Ana Susín, Rafael Torres-Masjoan, Laia Leyva-Díaz, Eduardo Karow, Marisa Figueres-Oñate, María López-Mascaraque, Laura López-Atalaya, José P. Berninger, Benedikt López-Bendito, Guillermina |
author_sort | Herrero-Navarro, Álvaro |
collection | PubMed |
description | Neural cell diversity is essential to endow distinct brain regions with specific functions. During development, progenitors within these regions are characterized by specific gene expression programs, contributing to the generation of diversity in postmitotic neurons and astrocytes. While the region-specific molecular diversity of neurons and astrocytes is increasingly understood, whether these cells share region-specific programs remains unknown. Here, we show that in the neocortex and thalamus, neurons and astrocytes express shared region-specific transcriptional and epigenetic signatures. These signatures not only distinguish cells across these two brain regions but are also detected across substructures within regions, such as distinct thalamic nuclei, where clonal analysis reveals the existence of common nucleus-specific progenitors for neurons and astrocytes. Consistent with their shared molecular signature, regional specificity is maintained following astrocyte-to-neuron reprogramming. A detailed understanding of these regional-specific signatures may thus inform strategies for future cell-based brain repair. |
format | Online Article Text |
id | pubmed-8026135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80261352021-04-21 Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming Herrero-Navarro, Álvaro Puche-Aroca, Lorenzo Moreno-Juan, Verónica Sempere-Ferràndez, Alejandro Espinosa, Ana Susín, Rafael Torres-Masjoan, Laia Leyva-Díaz, Eduardo Karow, Marisa Figueres-Oñate, María López-Mascaraque, Laura López-Atalaya, José P. Berninger, Benedikt López-Bendito, Guillermina Sci Adv Research Articles Neural cell diversity is essential to endow distinct brain regions with specific functions. During development, progenitors within these regions are characterized by specific gene expression programs, contributing to the generation of diversity in postmitotic neurons and astrocytes. While the region-specific molecular diversity of neurons and astrocytes is increasingly understood, whether these cells share region-specific programs remains unknown. Here, we show that in the neocortex and thalamus, neurons and astrocytes express shared region-specific transcriptional and epigenetic signatures. These signatures not only distinguish cells across these two brain regions but are also detected across substructures within regions, such as distinct thalamic nuclei, where clonal analysis reveals the existence of common nucleus-specific progenitors for neurons and astrocytes. Consistent with their shared molecular signature, regional specificity is maintained following astrocyte-to-neuron reprogramming. A detailed understanding of these regional-specific signatures may thus inform strategies for future cell-based brain repair. American Association for the Advancement of Science 2021-04-07 /pmc/articles/PMC8026135/ /pubmed/33827819 http://dx.doi.org/10.1126/sciadv.abe8978 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Herrero-Navarro, Álvaro Puche-Aroca, Lorenzo Moreno-Juan, Verónica Sempere-Ferràndez, Alejandro Espinosa, Ana Susín, Rafael Torres-Masjoan, Laia Leyva-Díaz, Eduardo Karow, Marisa Figueres-Oñate, María López-Mascaraque, Laura López-Atalaya, José P. Berninger, Benedikt López-Bendito, Guillermina Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title | Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title_full | Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title_fullStr | Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title_full_unstemmed | Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title_short | Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
title_sort | astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026135/ https://www.ncbi.nlm.nih.gov/pubmed/33827819 http://dx.doi.org/10.1126/sciadv.abe8978 |
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