ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integri...

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Detalles Bibliográficos
Autores principales: García-Castro, Helena, Kenny, Nathan J., Iglesias, Marta, Álvarez-Campos, Patricia, Mason, Vincent, Elek, Anamaria, Schönauer, Anna, Sleight, Victoria A., Neiro, Jakke, Aboobaker, Aziz, Permanyer, Jon, Irimia, Manuel, Sebé-Pedrós, Arnau, Solana, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028764/
https://www.ncbi.nlm.nih.gov/pubmed/33827654
http://dx.doi.org/10.1186/s13059-021-02302-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028764/
https://www.ncbi.nlm.nih.gov/pubmed/33827654
http://dx.doi.org/10.1186/s13059-021-02302-5

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