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Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi
The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite for many standard experiments for the functional analysis of genes, including the generation of deletion mutants and the localization of gene products. Here, we provide Golden Gate vectors for fast and easy cl...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032637/ https://www.ncbi.nlm.nih.gov/pubmed/33367953 http://dx.doi.org/10.1007/s00294-020-01143-2 |