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Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi

The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite for many standard experiments for the functional analysis of genes, including the generation of deletion mutants and the localization of gene products. Here, we provide Golden Gate vectors for fast and easy cl...

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Detalles Bibliográficos
Autores principales: Dahlmann, Tim A., Terfehr, Dominik, Becker, Kordula, Teichert, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032637/
https://www.ncbi.nlm.nih.gov/pubmed/33367953
http://dx.doi.org/10.1007/s00294-020-01143-2