An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue

Biolistic delivery is widely used for genetic transformation but inconsistency between bombardment samples for transient gene expression analysis often hinders quantitative analyses. We developed a methodology to improve the consistency of biolistic delivery results by using a double-barrel device a...

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Detalles Bibliográficos
Autores principales: Miller, Kyle, Eggenberger, Alan L., Lee, Keunsub, Liu, Fei, Kang, Minjeong, Drent, Madison, Ruba, Andrew, Kirscht, Tyler, Wang, Kan, Jiang, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032657/
https://www.ncbi.nlm.nih.gov/pubmed/33833247
http://dx.doi.org/10.1038/s41598-021-86549-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032657/
https://www.ncbi.nlm.nih.gov/pubmed/33833247
http://dx.doi.org/10.1038/s41598-021-86549-9

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