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Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo

Various in vivo mutagenesis methods have been developed to facilitate fast and efficient continuous evolution of proteins in cells. However, they either modify the DNA region that does not match the target gene, or suffer from low mutation rates. Here, we report a mutator, eMutaT7 (enhanced MutaT7),...

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Detalles Bibliográficos
Autores principales: Park, Hyojin, Kim, Seokhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034631/
https://www.ncbi.nlm.nih.gov/pubmed/33406230
http://dx.doi.org/10.1093/nar/gkaa1231