Cargando…
Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo
Various in vivo mutagenesis methods have been developed to facilitate fast and efficient continuous evolution of proteins in cells. However, they either modify the DNA region that does not match the target gene, or suffer from low mutation rates. Here, we report a mutator, eMutaT7 (enhanced MutaT7),...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034631/ https://www.ncbi.nlm.nih.gov/pubmed/33406230 http://dx.doi.org/10.1093/nar/gkaa1231 |