Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects

Several existing technologies enable short genomic alterations including generating indels and short nucleotide variants, however, engineering more significant genomic changes is more challenging due to reduced efficiency and precision. Here, we developed RecT Editor via Designer-Cas9-Initiated Targ...

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Detalles Bibliográficos
Autores principales: Wang, Chengkun, Cheng, Jason K W, Zhang, Qianhe, Hughes, Nicholas W, Xia, Qiong, Winslow, Monte M, Cong, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034634/
https://www.ncbi.nlm.nih.gov/pubmed/33619540
http://dx.doi.org/10.1093/nar/gkaa1264

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034634/
https://www.ncbi.nlm.nih.gov/pubmed/33619540
http://dx.doi.org/10.1093/nar/gkaa1264

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