Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan

SIMPLE SUMMARY: Epstein–Barr virus DNA positivity, age ≥ 55 years, cigarette smoking, and high BCL-2, B2M, and CD161 expression were identified as independent risk factors for primary laryngeal squamous cell carcinoma. A high EBER signal and low CD3 expression significantly and independently predict...

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Autores principales: Lee, Li-Ang, Fang, Tuan-Jen, Li, Hsueh-Yu, Chuang, Hai-Hua, Kang, Chung-Jan, Chang, Kai-Ping, Liao, Chun-Ta, Chen, Tse-Ching, Huang, Chung-Guei, Yen, Tzu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038767/
https://www.ncbi.nlm.nih.gov/pubmed/33917480
http://dx.doi.org/10.3390/cancers13071741
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author Lee, Li-Ang
Fang, Tuan-Jen
Li, Hsueh-Yu
Chuang, Hai-Hua
Kang, Chung-Jan
Chang, Kai-Ping
Liao, Chun-Ta
Chen, Tse-Ching
Huang, Chung-Guei
Yen, Tzu-Chen
author_facet Lee, Li-Ang
Fang, Tuan-Jen
Li, Hsueh-Yu
Chuang, Hai-Hua
Kang, Chung-Jan
Chang, Kai-Ping
Liao, Chun-Ta
Chen, Tse-Ching
Huang, Chung-Guei
Yen, Tzu-Chen
author_sort Lee, Li-Ang
collection PubMed
description SIMPLE SUMMARY: Epstein–Barr virus DNA positivity, age ≥ 55 years, cigarette smoking, and high BCL-2, B2M, and CD161 expression were identified as independent risk factors for primary laryngeal squamous cell carcinoma. A high EBER signal and low CD3 expression significantly and independently predicted local recurrence and disease-free survival within five years. The information obtained in this study improves our understanding of viral infections in laryngeal cancer, and may guide future prevention, treatment, and follow-up strategies. ABSTRACT: Mounting molecular evidence supports Epstein–Barr virus (EBV) involvement in the pathogenesis of laryngeal squamous cell carcinoma (LSCC); however, the epidemiological data are inconsistent. In this retrospective case-control study, we aimed to determine whether EBV infection underlies the risk and prognosis of LSCC. The prevalence of EBV infection, as analyzed using an EBV DNA polymerase chain reaction assay, was significantly higher in 42 Taiwanese patients with newly diagnosed primary LSCC, compared to 39 age- and sex-matched control patients without cancer (48% vs. 19%). Furthermore, most of the EBER signals detected using in situ hybridization were localized to the nuclei of tumor-infiltrating lymphocytes. In multivariate analysis, EBV DNA positivity, age ≥ 55 years, cigarette smoking, and high BCL-2, B2M, and CD161 expression (assessed using immunohistochemistry) were identified as independent risk factors for LSCC. Furthermore, five-year local recurrence and disease-free survival rates were 34% and 58%, respectively, with a high EBER signal and low CD3 expression independently predicting five-year local recurrence and disease-free survival. Our comprehensive profiling data accurately identified patients at risk for LSCC development, local recurrence, or disease-free survival. The information obtained in this study improves our understanding of EBV infection in LSCC, and may guide precision medicine for patients with LSCC.
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spelling pubmed-80387672021-04-12 Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan Lee, Li-Ang Fang, Tuan-Jen Li, Hsueh-Yu Chuang, Hai-Hua Kang, Chung-Jan Chang, Kai-Ping Liao, Chun-Ta Chen, Tse-Ching Huang, Chung-Guei Yen, Tzu-Chen Cancers (Basel) Article SIMPLE SUMMARY: Epstein–Barr virus DNA positivity, age ≥ 55 years, cigarette smoking, and high BCL-2, B2M, and CD161 expression were identified as independent risk factors for primary laryngeal squamous cell carcinoma. A high EBER signal and low CD3 expression significantly and independently predicted local recurrence and disease-free survival within five years. The information obtained in this study improves our understanding of viral infections in laryngeal cancer, and may guide future prevention, treatment, and follow-up strategies. ABSTRACT: Mounting molecular evidence supports Epstein–Barr virus (EBV) involvement in the pathogenesis of laryngeal squamous cell carcinoma (LSCC); however, the epidemiological data are inconsistent. In this retrospective case-control study, we aimed to determine whether EBV infection underlies the risk and prognosis of LSCC. The prevalence of EBV infection, as analyzed using an EBV DNA polymerase chain reaction assay, was significantly higher in 42 Taiwanese patients with newly diagnosed primary LSCC, compared to 39 age- and sex-matched control patients without cancer (48% vs. 19%). Furthermore, most of the EBER signals detected using in situ hybridization were localized to the nuclei of tumor-infiltrating lymphocytes. In multivariate analysis, EBV DNA positivity, age ≥ 55 years, cigarette smoking, and high BCL-2, B2M, and CD161 expression (assessed using immunohistochemistry) were identified as independent risk factors for LSCC. Furthermore, five-year local recurrence and disease-free survival rates were 34% and 58%, respectively, with a high EBER signal and low CD3 expression independently predicting five-year local recurrence and disease-free survival. Our comprehensive profiling data accurately identified patients at risk for LSCC development, local recurrence, or disease-free survival. The information obtained in this study improves our understanding of EBV infection in LSCC, and may guide precision medicine for patients with LSCC. MDPI 2021-04-06 /pmc/articles/PMC8038767/ /pubmed/33917480 http://dx.doi.org/10.3390/cancers13071741 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Li-Ang
Fang, Tuan-Jen
Li, Hsueh-Yu
Chuang, Hai-Hua
Kang, Chung-Jan
Chang, Kai-Ping
Liao, Chun-Ta
Chen, Tse-Ching
Huang, Chung-Guei
Yen, Tzu-Chen
Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title_full Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title_fullStr Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title_full_unstemmed Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title_short Effects of Epstein-Barr Virus Infection on the Risk and Prognosis of Primary Laryngeal Squamous Cell Carcinoma: A Hospital-Based Case-Control Study in Taiwan
title_sort effects of epstein-barr virus infection on the risk and prognosis of primary laryngeal squamous cell carcinoma: a hospital-based case-control study in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038767/
https://www.ncbi.nlm.nih.gov/pubmed/33917480
http://dx.doi.org/10.3390/cancers13071741
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