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Protocol to produce high-titer retrovirus for transduction of mouse bone marrow cells

Regulating gene expression through retroviral infection has been widely used in mouse bone marrow transplantation (BMT) to test the capacity of self-renewal, as well as multi-lineage differentiation of hematopoietic stem and progenitor cells (HSPCs). However, it remains challenging to achieve high t...

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Detalles Bibliográficos
Autores principales: Qu, Jing, Yang, Zhenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038942/
https://www.ncbi.nlm.nih.gov/pubmed/33851142
http://dx.doi.org/10.1016/j.xpro.2021.100459