Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24...

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Detalles Bibliográficos
Autores principales: Gotoh, Kazuhito, Takata, Yurie, Nakashima, Yuya, Mizuguchi, Soichi, Komori, Keishi, Kang, Dongchon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039729/
https://www.ncbi.nlm.nih.gov/pubmed/33851138
http://dx.doi.org/10.1016/j.xpro.2021.100401

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039729/
https://www.ncbi.nlm.nih.gov/pubmed/33851138
http://dx.doi.org/10.1016/j.xpro.2021.100401

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