Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24...

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Autores principales: Gotoh, Kazuhito, Takata, Yurie, Nakashima, Yuya, Mizuguchi, Soichi, Komori, Keishi, Kang, Dongchon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039729/
https://www.ncbi.nlm.nih.gov/pubmed/33851138
http://dx.doi.org/10.1016/j.xpro.2021.100401
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author Gotoh, Kazuhito
Takata, Yurie
Nakashima, Yuya
Mizuguchi, Soichi
Komori, Keishi
Kang, Dongchon
author_facet Gotoh, Kazuhito
Takata, Yurie
Nakashima, Yuya
Mizuguchi, Soichi
Komori, Keishi
Kang, Dongchon
author_sort Gotoh, Kazuhito
collection PubMed
description Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
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spelling pubmed-80397292021-04-12 Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer Gotoh, Kazuhito Takata, Yurie Nakashima, Yuya Mizuguchi, Soichi Komori, Keishi Kang, Dongchon STAR Protoc Protocol Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018). Elsevier 2021-03-26 /pmc/articles/PMC8039729/ /pubmed/33851138 http://dx.doi.org/10.1016/j.xpro.2021.100401 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Gotoh, Kazuhito
Takata, Yurie
Nakashima, Yuya
Mizuguchi, Soichi
Komori, Keishi
Kang, Dongchon
Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title_full Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title_fullStr Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title_full_unstemmed Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title_short Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
title_sort metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039729/
https://www.ncbi.nlm.nih.gov/pubmed/33851138
http://dx.doi.org/10.1016/j.xpro.2021.100401
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