Dissociation of chick embryonic tissue for FACS and preparation of isolated cells for genome-wide downstream assays

In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an optimized...

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Detalles Bibliográficos
Autores principales: Williams, Ruth M., Sauka-Spengler, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039852/
https://www.ncbi.nlm.nih.gov/pubmed/33870222
http://dx.doi.org/10.1016/j.xpro.2021.100414

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039852/
https://www.ncbi.nlm.nih.gov/pubmed/33870222
http://dx.doi.org/10.1016/j.xpro.2021.100414

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