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Dissociation of chick embryonic tissue for FACS and preparation of isolated cells for genome-wide downstream assays
In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an optimized...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039852/ https://www.ncbi.nlm.nih.gov/pubmed/33870222 http://dx.doi.org/10.1016/j.xpro.2021.100414 |