Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids

The S9.6 antibody is broadly used to detect RNA:DNA hybrids but has significant affinity for double-stranded RNA. The impact of this off-target RNA binding activity has not been thoroughly investigated, especially in the context of immunofluorescence microscopy. We report that S9.6 immunofluorescenc...

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Detalles Bibliográficos
Autores principales: Smolka, John A., Sanz, Lionel A., Hartono, Stella R., Chédin, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2021
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040515/
https://www.ncbi.nlm.nih.gov/pubmed/33830170
http://dx.doi.org/10.1083/jcb.202004079

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040515/
https://www.ncbi.nlm.nih.gov/pubmed/33830170
http://dx.doi.org/10.1083/jcb.202004079

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