A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augment a mini-Mu transposon-ba...

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Detalles Bibliográficos
Autores principales: Ho, Trevor Y. H., Shao, Alexander, Lu, Zeyu, Savilahti, Harri, Menolascina, Filippo, Wang, Lei, Dalchau, Neil, Wang, Baojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044194/
https://www.ncbi.nlm.nih.gov/pubmed/33850130
http://dx.doi.org/10.1038/s41467-021-22404-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044194/
https://www.ncbi.nlm.nih.gov/pubmed/33850130
http://dx.doi.org/10.1038/s41467-021-22404-9

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