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A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augment a mini-Mu transposon-ba...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044194/ https://www.ncbi.nlm.nih.gov/pubmed/33850130 http://dx.doi.org/10.1038/s41467-021-22404-9 |