Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melted initiat...

Descripción completa

Detalles Bibliográficos
Autores principales: Martinez, Sergio E., Singh, Anupam, De Wijngaert, Brent, Sultana, Shemaila, Dharia, Chhaya, Vanbuel, Hans, Shen, Jiayu, Vasilchuk, Daniel, Patel, Smita S., Das, Kalyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044712/
https://www.ncbi.nlm.nih.gov/pubmed/33870232
http://dx.doi.org/10.1016/j.xpro.2021.100431

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044712/
https://www.ncbi.nlm.nih.gov/pubmed/33870232
http://dx.doi.org/10.1016/j.xpro.2021.100431

Ejemplares similares