Cargando…

Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melted initiat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Martinez, Sergio E., Singh, Anupam, De Wijngaert, Brent, Sultana, Shemaila, Dharia, Chhaya, Vanbuel, Hans, Shen, Jiayu, Vasilchuk, Daniel, Patel, Smita S., Das, Kalyan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044712/ https://www.ncbi.nlm.nih.gov/pubmed/33870232 http://dx.doi.org/10.1016/j.xpro.2021.100431

Ejemplares similares

Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit
por: Li, Ningning, et al.
Publicado: (2013)

Structures illustrate step-by-step mitochondrial transcription initiation
por: Goovaerts, Quinten, et al.
Publicado: (2023)

Cryo-EM structures of wild-type and E138K/M184I mutant HIV-1 RT/DNA complexed with inhibitors doravirine and rilpivirine
por: Singh, Abhimanyu K., et al.
Publicado: (2022)

Cryo-EM structures of human RNA polymerase I
por: Misiaszek, Agata D., et al.
Publicado: (2021)

Cryo-EM visualization of DNA-PKcs structural intermediates in NHEJ
por: Chen, Siyu, et al.
Publicado: (2023)

Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
por: Zhou, Dejian, et al.
Publicado: (2018)

Cryo-EM structure of the respiratory syncytial virus RNA polymerase
por: Cao, Dongdong, et al.
Publicado: (2020)

Conformational landscape of the yeast SAGA complex as revealed by cryo-EM
por: Vasyliuk, Diana, et al.
Publicado: (2022)

Cryo-EM of the dynamin polymer assembled on lipid membrane
por: Kong, Leopold, et al.
Publicado: (2018)

Cryo-EM reveals the asymmetric assembly of squid hemocyanin
por: Tanaka, Yoshikazu, et al.
Publicado: (2019)

Bayesian Modeling of Biomolecular Assemblies with Cryo-EM Maps
por: Habeck, Michael
Publicado: (2017)

CryoEM structure of the Nipah virus nucleocapsid assembly
por: Ker, De-Sheng, et al.
Publicado: (2021)

Cryo-EM structure of the fully assembled Elongator complex
por: Jaciuk, Marcin, et al.
Publicado: (2023)

Cryo-EM captures early ribosome assembly in action
por: Qin, Bo, et al.
Publicado: (2023)

Cryo-EM reveals conformational flexibility in apo DNA polymerase ζ
por: Du Truong, Chloe, et al.
Publicado: (2021)

Helicase promotes replication re-initiation from an RNA transcript
por: Sun, Bo, et al.
Publicado: (2018)

EMatch: an efficient method for aligning atomic resolution subunits into intermediate-resolution cryo-EM maps of large macromolecular assemblies
por: Dror, Oranit, et al.
Publicado: (2007)

Model building of protein complexes from intermediate-resolution cryo-EM maps with deep learning-guided automatic assembly
por: He, Jiahua, et al.
Publicado: (2022)

Structural differences between yeast and mammalian microtubules revealed by cryo-EM
por: Howes, Stuart C., et al.
Publicado: (2017)

Assessment of protein–protein interfaces in cryo-EM derived assemblies
por: Malhotra, Sony, et al.
Publicado: (2021)

Cryo-EM analysis of Ebola virus nucleocapsid-like assembly
por: Wang, Yan, et al.
Publicado: (2021)

Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate
por: Trask, Shane D., et al.
Publicado: (2010)

Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome
por: Armache, Jean Paul, et al.
Publicado: (2019)

Cryo‐EM structure of fission yeast tetrameric α‐mannosidase Ams1
por: Zhang, Jianxiu, et al.
Publicado: (2020)

Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
por: Dendooven, Tom, et al.
Publicado: (2023)

Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states
por: Girbig, Mathias, et al.
Publicado: (2021)

Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2
por: Fianu, Isaac, et al.
Publicado: (2021)

Cryo-EM structure of the nucleocapsid-like assembly of respiratory syncytial virus
por: Wang, Yan, et al.
Publicado: (2023)

Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation
por: Hansen, Jesse M, et al.
Publicado: (2021)

Cryo-EM structure of the plant 26S proteasome
por: Kandolf, Susanne, et al.
Publicado: (2022)

Protein Secondary Structure Detection in Intermediate Resolution Cryo-EM Maps Using Deep Learning
por: Subramaniya, Sai Raghavendra Maddhuri Venkata, et al.
Publicado: (2019)

Cryo-EM structures of intermediates suggest an alternative catalytic reaction cycle for cytochrome c oxidase
por: Kolbe, F., et al.
Publicado: (2021)

Cryo-EM structures of alphavirus conformational intermediates in low pH–triggered prefusion states
por: Chen, Chun-Liang, et al.
Publicado: (2022)

Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa
por: Zhang, Xu, et al.
Publicado: (2023)

A snapshot of cryo‐EM
por: Skiniotis, Georgios
Publicado: (2016)

Interpreting the cryo-EM map
por: Rosenthal, Peter B.
Publicado: (2019)

COVID-19 and cryo-EM
por: Subramaniam, Sriram
Publicado: (2020)

Cryo-EM at ACA 2022
por: Subramaniam, Sriram, et al.
Publicado: (2022)

Cryo-EM structure of translesion DNA synthesis polymerase ζ with a base pair mismatch
por: Malik, Radhika, et al.
Publicado: (2022)

A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex
por: Zhang, Hong-Wei, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_gj9pf6oqflcvkip990jdvsb4em