Modified PCR protocol to increase sensitivity for determination of bacterial community composition

BACKGROUND: The objective of this project was to increase the sensitivity of sequence-based bacterial community determination without impacting community composition or interfering with cluster formation during sequencing. Two PCR protocols (standard and modified) were examined in airway samples whe...

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Detalles Bibliográficos
Autores principales: Williamson, Kayla M., Wagner, Brandie D., Robertson, Charles E., Stevens, Mark J., Sontag, Marci K., Mourani, Peter M., Harris, J. Kirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045227/
https://www.ncbi.nlm.nih.gov/pubmed/33849648
http://dx.doi.org/10.1186/s40168-020-00958-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045227/
https://www.ncbi.nlm.nih.gov/pubmed/33849648
http://dx.doi.org/10.1186/s40168-020-00958-y

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