CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering

CYP154C5 from Nocardia farcinica is a P450 monooxygenase able to hydroxylate a range of steroids with high regio‐ and stereoselectivity at the 16α‐position. Using protein engineering and substrate modifications based on the crystal structure of CYP154C5, an altered regioselectivity of the enzyme in...

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Detalles Bibliográficos
Autores principales: Bracco, Paula, Wijma, Hein J., Nicolai, Bastian, Buitrago, Jhon Alexander Rodriguez, Klünemann, Thomas, Vila, Agustina, Schrepfer, Patrick, Blankenfeldt, Wulf, Janssen, Dick B., Schallmey, Anett
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048783/
https://www.ncbi.nlm.nih.gov/pubmed/33145893
http://dx.doi.org/10.1002/cbic.202000735

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048783/
https://www.ncbi.nlm.nih.gov/pubmed/33145893
http://dx.doi.org/10.1002/cbic.202000735

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