The V(H) framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants

In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting “hot regions,” i.e., amino acid substitutions therein...

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Detalles Bibliográficos
Autores principales: Kiguchi, Yuki, Oyama, Hiroyuki, Morita, Izumi, Nagata, Yasuhiro, Umezawa, Naoko, Kobayashi, Norihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050046/
https://www.ncbi.nlm.nih.gov/pubmed/33859250
http://dx.doi.org/10.1038/s41598-021-87501-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050046/
https://www.ncbi.nlm.nih.gov/pubmed/33859250
http://dx.doi.org/10.1038/s41598-021-87501-7

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