Ultrasensitive nucleic acid detection based on phosphorothioated hairpin-assisted isothermal amplification

Herein, we describe a phosphorothioated hairpin-assisted isothermal amplification (PHAmp) method for detection of a target nucleic acid. The hairpin probe (HP) is designed to contain a 5′ phosphorothioate (PS)-modified overhang, a target recognition site, and a 3′ self-priming (SP) region. Upon bind...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Yujin, Song, Jayeon, Park, Hyun Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052315/
https://www.ncbi.nlm.nih.gov/pubmed/33863981
http://dx.doi.org/10.1038/s41598-021-87948-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052315/
https://www.ncbi.nlm.nih.gov/pubmed/33863981
http://dx.doi.org/10.1038/s41598-021-87948-8

Ejemplares similares