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Ultrasensitive nucleic acid detection based on phosphorothioated hairpin-assisted isothermal amplification
Herein, we describe a phosphorothioated hairpin-assisted isothermal amplification (PHAmp) method for detection of a target nucleic acid. The hairpin probe (HP) is designed to contain a 5′ phosphorothioate (PS)-modified overhang, a target recognition site, and a 3′ self-priming (SP) region. Upon bind...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052315/ https://www.ncbi.nlm.nih.gov/pubmed/33863981 http://dx.doi.org/10.1038/s41598-021-87948-8 |