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A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing

Generation of conditional knockout (cKO) and various gene-modified cells is laborious and time-consuming. Here, we established an all-in-one cKO system, which enables highly efficient generation of cKO cells and simultaneous gene modifications, including epitope tagging and reporter gene knock-in. W...

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Detalles Bibliográficos
Autores principales:	Suzuki, Teruhiko, Katada, Eiji, Mizuoka, Yuki, Takagi, Satoko, Kazuki, Yasuhiro, Oshimura, Mitsuo, Shindo, Mayumi, Hara, Takahiko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053084/ https://www.ncbi.nlm.nih.gov/pubmed/33503245 http://dx.doi.org/10.1093/nar/gkaa1296

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