Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing

Modifications in RNA are numerous (∼170) and in higher numbers compared to DNA (∼5) making the ability to sequence an RNA molecule to identify these modifications highly tenuous using next generation sequencing (NGS). The ability to immobilize an exoribonuclease enzyme, such as XRN1, to a solid supp...

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Detalles Bibliográficos
Autores principales: Athapattu, Uditha S, Amarasekara, Charuni A, Immel, Jacob R, Bloom, Steven, Barany, Francis, Nagel, Aaron C, Soper, Steven A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053086/
https://www.ncbi.nlm.nih.gov/pubmed/33511416
http://dx.doi.org/10.1093/nar/gkab001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053086/
https://www.ncbi.nlm.nih.gov/pubmed/33511416
http://dx.doi.org/10.1093/nar/gkab001

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